Doctors Without Borders (MSF) says it has been forced to end migrant rescue operations in the Mediterranean carried out by the vessel Aquarius.
The medical charity blamed sustained attacks on search and rescue by European states.
Italy's Interior Minister Matteo Salvini welcomed MSF's move. Fewer sailings, fewer landings, fewer deaths. That's good, he tweeted.
Aquarius has been stuck in Marseille since its registration was revoked.
It has helped save migrants making the dangerous crossing to Europe from Libya and elsewhere, but has faced strong opposition, particularly from Italy.
Mr Salvini accused charities running rescue ships of collaborating with people-traffickers operating out of Libya to run a taxi service to Italian ports.
Italian policy is that migrants picked up at sea should be returned to Libya by that country's coastguard.
But charities and human rights groups say migrants face appalling conditions in Libya, where abuses at the hands of people-trafficking gangs are rife.
Aquarius had been the last charity rescue ship still operating.
Announcing the decision to end its operations, MSF said EU countries, spearheaded by Italy, had failed to provide enough dedicated rescue capacity of their own, then had actively sabotaged the efforts of others trying to save lives in the Mediterranean, the BBC's Europe correspondent, Damian Grammaticas reports.
In a tweet, MSF Sea said sustained attacks by European nations will mean more deaths at sea, and more needless deaths that will go unwitnessed.
Aquarius has been laid up in Marseille for months, after Panama revoked its registration citing pressure from Italian authorities
Source: Voice of Nigeria